catalog number :
MBS721506
products type :
ELISA Kit
products full name :
Human Latent membrane protein 1 ELISA Kit
products short name :
Latent membrane protein 1
other names :
Protein LMP-1; LAMP family protein lmp-1; Protein LMP-1
products gene name :
LMP 1
other gene names :
lmp-1; lmp-1; C03B1.12
uniprot entry name :
LMP1_CAEEL
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This LMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: LMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LMP-1 antibody and an LMP-1-HRP conjugate. The assay sample and buffer are incubated together with LMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LMP-1 concentration since LMP-1 from samples and LMP-1-HRP conjugate compete for the anti-LMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LMP-1 from the sample, fewer sites are left to bind LMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LMP-1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_509072.1
ncbi gb acc num :
NM_076671.4
ncbi mol weight :
25,792 Da
ncbi pathways :
Lysosome Pathway (96893); Lysosome Pathway (96865); Phagosome Pathway (153861); Phagosome Pathway (153859)