ELISA/assay

Rat Acetyl Coenzyme A Carboxylase Alpha ELISA Kit

MBS721488

48-Strip-Wells

470 USD

ELISA Kit

Rat Acetyl Coenzyme A Carboxylase Alpha ELISA Kit

Acetyl Coenzyme A Carboxylase Alpha

ACACA protein, partial; ACACA protein; ACACA protein

ACACa

ACACA

Q7Z5W8_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ACCalpha. No significant cross-reactivity or interference between ACCalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACCalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This ACCalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ACCalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: ACCalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACCalpha. Standards or samples are then added to the microtiter plate wells and ACCalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACCalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACCalpha are added to each well to "sandwich" the ACCalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACCalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACCalpha concentration in each sample is interpolated from this standard curve.

32425437

AAH31485.1

78,272 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Biotin Transport And Metabolism Pathway (106257); ChREBP Activates Metabolic Gene Expression Pathway (106104); Corticotropin-releasing Hormone Pathway (920957); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012)

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. Protein type: EC 6.4.1.2; EC 6.3.4.14; Carbohydrate Metabolism - propanoate; Lipid Metabolism - fatty acid biosynthesis; Carbohydrate Metabolism - pyruvate; Ligase. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton. Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding. Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; multicellular organismal protein metabolic process; tissue homeostasis; positive regulation of cellular metabolic process; energy reserve metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; cellular lipid metabolic process; carnitine shuttle; water-soluble vitamin metabolic process; fatty acid biosynthetic process; biotin metabolic process; protein homotetramerization. Disease: Acetyl-coa Carboxylase Deficiency

48-Strip-Wells

470 USD

96-Strip-Wells

675