ELISA/assay

Human DNA binding protein inhibitor ID 2 (ID2) ELISA Kit

MBS7214645

48-Strip-Wells

470 USD

ELISA Kit

Human DNA binding protein inhibitor ID 2 (ID2) ELISA Kit

DNA binding protein inhibitor ID 2 (ID2)

DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID-2; helix-loop-helix protein ID2; cell growth-inhibiting gene 8; inhibitor of differentiation 2; DNA-binding protein inhibitor ID2; class B basic helix-loop-helix protein 26; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; Class B basic helix-loop-helix protein 26; bHLHb26; Inhibitor of DNA binding 2; Inhibitor of differentiation 2

ID2

ID2; ID2; GIG8; ID2A; ID2H; bHLHb26; BHLHB26; bHLHb26

ID2_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ID2. No significant cross-reactivity or interference between ID2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ID2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ID2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ID2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: ID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ID2 antibody and an ID2-HRP conjugate. The assay sample and buffer are incubated together with ID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ID2 concentration since ID2 from samples and ID2-HRP conjugate compete for the anti-ID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ID2 from the sample, fewer sites are left to bind ID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ID2 concentration in each sample is interpolated from this standard curve.

31982933

NP_002157.2

NM_002166.4

14,917 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Id Signaling Pathway (198871); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475); Transcriptional Misregulation In Cancer Pathway (523016)

The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]

ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system. Protein type: DNA-binding; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: nucleoplasm; microtubule cytoskeleton; centrosome; protein complex; cytoplasm; nucleus; chromatin; cytosol. Molecular Function: protein dimerization activity; protein binding. Biological Process: regulation of lipid metabolic process; positive regulation of smooth muscle cell proliferation; multicellular organismal development; positive regulation of transcription, DNA-dependent; cell maturation; negative regulation of transcription from RNA polymerase II promoter; entrainment of circadian clock by photoperiod; locomotor rhythm; natural killer cell differentiation; embryonic digestive tract morphogenesis; negative regulation of osteoblast differentiation; circadian regulation of gene expression; oligodendrocyte development; positive regulation of macrophage differentiation; positive regulation of astrocyte differentiation; negative regulation of B cell differentiation; Peyer s patch development; transcription, DNA-dependent; negative regulation of transcription factor activity; olfactory bulb development; positive regulation of erythrocyte differentiation; neuron fate commitment; mammary gland epithelial cell proliferation; enucleate erythrocyte differentiation; regulation of circadian rhythm; negative regulation of DNA binding; negative regulation of oligodendrocyte differentiation; negative regulation of neuron differentiation; positive regulation of fat cell differentiation; negative regulation of transcription, DNA-dependent; metanephros development; positive regulation of blood pressure

48-Strip-Wells

470 USD

96-Strip-Wells

675