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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat alpha1 Antitrypsin ELISA Kit
catalog :
MBS721462
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS721462
products type :
ELISA Kit
products full name :
Rat alpha1 Antitrypsin ELISA Kit
products short name :
alpha1 Antitrypsin
products name syn :
Rat a1 Antitrypsin ELISA Kit
other names :
alpha1-antitrypsin; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-proteinase inhibitor
products gene name :
alpha1-AT
products gene name syn :
a1-AT
uniprot entry name :
A1AT_CALCN
reactivity :
Rat
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This alpha1AT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat alpha1AT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the Assay: alpha1AT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha1AT antibody and an alpha1AT-HRP conjugate. The assay sample and buffer are incubated together with alpha1AT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha1AT concentration since alpha1AT from samples and alpha1AT-HRP conjugate compete for the anti-alpha1AT antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha1AT from the sample, fewer sites are left to bind alpha1AT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1AT concentration in each sample is interpolated from this standard curve.
ncbi gi num :
2804195
ncbi acc num :
BAA24422.1
ncbi mol weight :
45,729 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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