ELISA/assay

Human Angiopoietin Growth Factors ELISA Kit

MBS721448

48-Strip-Wells

470 USD

ELISA Kit

Human Angiopoietin Growth Factors ELISA Kit

Angiopoietin Growth Factors

angiopoietin 1; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1

AGF

ANGPT1; ANGPT1; AGP1; AGPT; ANG1; KIAA0003; ANG-1

ANGP1_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Cardiovascular

INTENDED USE: This GSTa1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GSTa1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY: GSTa1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GSTa1. Standards or samples are then added to the microtiter plate wells and GSTa1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GSTa1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GSTa1 are added to each well to “sandwich” the GSTa1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GSTa1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSTa1 concentration in each sample is interpolated from this standard curve.

22203641

AAM92271.1

57,456 Da

Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; microvillus; extracellular region; plasma membrane; lipid raft. Molecular Function: receptor tyrosine kinase binding. Biological Process: positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; negative regulation of protein amino acid phosphorylation; positive chemotaxis; hemopoiesis; negative regulation of neuron apoptosis; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of endothelial cell proliferation; Tie receptor signaling pathway; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; blood coagulation; leukocyte migration; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675