ELISA/assay

Rat Growth Hormone Binding Protein ELISA Kit

MBS721432

96 Strip Wells

675 USD

ELISA Kit

Rat Growth Hormone Binding Protein ELISA Kit

Growth Hormone Binding Protein

glucagon; Glucagon; glucagon; glucagon<

GHBP

GCG; GCG; GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2

GLUC_BOVIN

Rat

Store all reagents at 2-8 degree C.

Intended Uses: This GLUCAGON ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GLUCAGON in the sample, this GLUCAGON ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GLUCAGON concentration. The concentration of GLUCAGON in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This GLUCAGON enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GLUCAGON. Standards or samples are then added to the microtiter plate wells and GLUCAGON if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GLUCAGON present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GLUCAGON are added to each well to "sandwich" the GLUCAGON immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GLUCAGON and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

27806879

NP_776341.1

NM_173916.3

20,944 Da

Class B/2 (Secretin Family Receptors) Pathway 1008578!!G Alpha (q) Signalling Events Pathway 1008586!!G Alpha (s) Signalling Events Pathway 1008583!!GPCR Downstream Signaling Pathway 1008582!!GPCR Ligand Binding Pathway 1008553!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 1008603!!Glucagon Signaling In Metabolic Regulation Pathway 1009383!!Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway 1009382!!Glucagon-type Ligand Receptors Pathway 1008580!!Incretin Synthesis, Secretion, And Inactivation Pathway 1009571

96 Strip Wells

675 USD