ELISA/assay

Human Amylin ELISA Kit

MBS721421

48-Strip-Wells

470 USD

ELISA Kit

Human Amylin ELISA Kit

Amylin

amylin, partial; Islet amyloid polypeptide; islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin); islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide

Amylin

IAPP; IAPP; DAP; IAP; DAP

IAPP_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This Amy ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Amy. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: Amy ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Amy antibody and an Amy-HRP conjugate. The assay sample and buffer are incubated together with Amy-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Amy concentration since Amy from samples and Amy-HRP conjugate compete for the anti-Amy antibody binding site. Since the number of sites is limited, as more sites are occupied by Amy from the sample, fewer sites are left to bind Amy-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Amy concentration in each sample is interpolated from this standard curve.

457131

AAA35524.1

9,806 Da

Amyloids Pathway (366238); Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Developmental Biology Pathway (477129); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extracellular space; cell soma; extracellular region. Molecular Function: identical protein binding; hormone activity; receptor binding. Biological Process: negative regulation of cell differentiation; cell-cell signaling; eating behavior; apoptosis; signal transduction; negative regulation of bone resorption; sensory perception of pain; endocrine pancreas development

48-Strip-Wells

470 USD

96-Strip-Wells

675