ELISA/assay

Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit

MBS7214137

48-Strip-Wells

470 USD

ELISA Kit

Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit

Retinoid isomerohydrolase (RPE65)

retinoid isomerohydrolase; Retinoid isomerohydrolase; retinoid isomerohydrolase; retinol isomerase; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase

RPE65

RPE65; RPE65

RPE65_CHICK

Mouse

This assay has high sensitivity and excellent specificity for detection of RPE65. No significant cross-reactivity or interference between RPE65 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPE65 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This RPE65 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RPE65. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay RPE65 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPE65 antibody and an RPE65-HRP conjugate. The assay sample and buffer are incubated together with RPE65-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPE65 concentration since RPE65 from samples and RPE65-HRP conjugate compete for the anti-RPE65 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPE65 from the sample, fewer sites are left to bind RPE65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPE65 concentration in each sample is interpolated from this standard curve.

45385809

NP_990215.1

NM_204884.1

60,930 Da

Retinol Metabolism Pathway (83930); Retinol Metabolism Pathway (406); Retinol Metabolism (BiGCaT, NuGO) Pathway (672480)

48-Strip-Wells

470 USD

96-Strip-Wells

675