ELISA/assay

Rat Monoamine oxidase A ELISA Kit

MBS721413

48-Strip-Wells

470 USD

ELISA Kit

Rat Monoamine oxidase A ELISA Kit

Monoamine oxidase A

monoamine oxidase A, partial; Amine oxidase [flavin-containing] A; amine oxidase [flavin-containing] A; amine oxidase [flavin-containing] A; monoamine oxidase type A; monoamine oxidase A; Monoamine oxidase type A; MAO-A

MAOA

MAOA; MAOA; MAO-A; MAO-A

AOFA_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of MOA. No significant cross-reactivity or interference between MOA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MOA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: MOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MOA antibody and an MOA-HRP conjugate. The assay sample and buffer are incubated together with MOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MOA concentration since MOA from samples and MOA-HRP conjugate compete for the anti-MOA antibody binding site. Since the number of sites is limited, as more sites are occupied by MOA from the sample, fewer sites are left to bind MOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MOA concentration in each sample is interpolated from this standard curve.

4262061

AAD14361.1

44,848 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amine Oxidase Reactions Pathway (105712); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biogenic Amine Synthesis Pathway (198793); Biological Oxidations Pathway (105698); Cocaine Addiction Pathway (546258)

This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family. Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Oxidoreductase; EC 1.4.3.4; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tryptophan; Membrane protein, integral; Amino Acid Metabolism - histidine; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: Xp11.3. Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane. Molecular Function: amine oxidase activity. Biological Process: synaptic transmission; biogenic amine metabolic process; neurotransmitter catabolic process; dopamine catabolic process; neurotransmitter secretion; xenobiotic metabolic process; neurotransmitter biosynthetic process. Disease: Brunner Syndrome

48-Strip-Wells

470 USD

96-Strip-Wells

675