ELISA/assay

Human Filamin C, Gamma ELISA Kit

MBS721356

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Filamin C, Gamma ELISA Kit

Filamin C, Gamma

filamin-C; Filamin-C; filamin-C; ABP-L; FLN-C; filamin-2; gamma-filamin; filamin, gamma; ABP-280-like protein; actin binding protein 280; actin-binding protein 280; actin-binding-like protein; filamin C, gamma; ABP-280-like protein; ABP-L; Actin-binding-like protein; Filamin-2; Gamma-filamin

FLNC

Flnc; Flnc; ABPL; Fln2; ABP-280; 1110055E19Rik; Abpl; Fln2; FLN-C

FLNC_MOUSE

Human

This assay has high sensitivity and excellent specificity for detection of FLNC. No significant cross-reactivity or interference between FLNC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FLNC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This FLNC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FLNC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: FLNC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FLNC. Standards or samples are then added to the microtiter plate wells and FLNC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FLNC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FLNC are added to each well to "sandwich" the FLNC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FLNC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLNC concentration in each sample is interpolated from this standard curve.

124487139

NP_001074654.1

NM_001081185.1

287,365 Da

Cell Junction Organization Pathway (1001003); Cell-Cell Communication Pathway (1001002); Cell-extracellular Matrix Interactions Pathway (1001008); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83245); MAPK Signaling Pathway (456); Proteoglycans In Cancer Pathway (782024); Proteoglycans In Cancer Pathway (782054); Salmonella Infection Pathway (375174)

FLNC: Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross- linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers. Defects in FLNC are the cause of myopathy myofibrillar type 5 (MFM5). A neuromuscular disorder, usually with an adult onset, characterized by focal myofibrillar destruction and pathological cytoplasmic protein aggregations, and clinical features of a limb-girdle myopathy. Defects in FLNC are the cause of myopathy distal type 4 (MPD4). MPD4 is a slowly progressive muscular disorder characterized by distal muscle weakness and atrophy affecting the upper and lower limbs. Onset occurs around the third to fourth decades of life, and patients remain ambulatory even after long disease duration. Muscle biopsy shows non-specific changes with no evidence of rods, necrosis, or inflammation. Belongs to the filamin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Cellular Component: cytoskeleton; focal adhesion; membrane; cytoplasm; plasma membrane; sarcoplasm; sarcolemma; actin cytoskeleton. Molecular Function: actin filament binding; cytoskeletal protein binding; ankyrin binding; actin binding. Biological Process: actin filament-based process; muscle fiber development

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675