Human Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit | MyBioSource MBS7213388 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit

catalog :

MBS7213388

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7213388

products type :

ELISA Kit

products full name :

Human Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit

products short name :

Serum paraoxonase/arylesterase 2 (PON2)

other names :

serum paraoxonase/arylesterase 2 isoform 2; Serum paraoxonase/arylesterase 2; serum paraoxonase/arylesterase 2; PON 2; A-esterase 2; paraoxonase nirs; aromatic esterase 2; serum aryldialkylphosphatase 2; paraoxonase 2; Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2

products gene name :

PON2

other gene names :

PON2; PON2; PON 2; A-esterase 2

uniprot entry name :

PON2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of PON2. No significant cross-reactivity or interference between PON2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PON2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This PON2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PON2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: PON2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PON2 antibody and an PON2-HRP conjugate. The assay sample and buffer are incubated together with PON2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PON2 concentration since PON2 from samples and PON2-HRP conjugate compete for the anti-PON2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PON2 from the sample, fewer sites are left to bind PON2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PON2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

66529396

ncbi acc num :

NP_001018171.1

ncbi gb acc num :

NM_001018161.1

ncbi mol weight :

37,996 Da

ncbi pathways :

Metabolic Pathways (132956); Phase I, Non P450 Pathway (198854)

ncbi summary :

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.1.1.81; Motility/polarity/chemotaxis; EC 3.1.1.2. Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: mitochondrion; lysosome; plasma membrane; extracellular region; nucleus. Molecular Function: identical protein binding; arylesterase activity; metal ion binding. Biological Process: response to oxidative stress; aromatic compound catabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage