ELISA/assay

Mouse C-C chemokine receptor type 9 (CCR9) ELISA Kit

MBS7213227

48-Strip-Wells

470 USD

ELISA Kit

Mouse C-C chemokine receptor type 9 (CCR9) ELISA Kit

C-C chemokine receptor type 9 (CCR9)

C-C chemokine receptor type 9 isoform B; C-C chemokine receptor type 9; C-C chemokine receptor type 9; G protein-coupled receptor 28; chemokine (C-C motif) receptor 9; G-protein coupled receptor 28; GPR-9-6; CD_antigen: CDw199

CCR9

CCR9; CCR9; GPR28; CDw199; GPR-9-6; CC-CKR-9; GPR28; C-C CKR-9; CC-CKR-9; CCR-9

CCR9_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CCR-9. No significant cross-reactivity or interference between CCR-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCR-9 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CCR-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CCR-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CCR-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCR-9 antibody and an CCR-9-HRP conjugate. The assay sample and buffer are incubated together with CCR-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCR-9 concentration since CCR-9 from samples and CCR-9-HRP conjugate compete for the anti-CCR-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCR-9 from the sample, fewer sites are left to bind CCR-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCR-9 concentration in each sample is interpolated from this standard curve.

373838705

NP_001243298.1

NM_001256369.1

40,713 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

CCR9: Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: C-C chemokine receptor activity; chemokine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cellular defense response; immune response; chemotaxis

48-Strip-Wells

470 USD

96-Strip-Wells

675