Human CD30 Ligand ELISA Kit | MyBioSource MBS721319 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CD30 Ligand ELISA Kit

catalog :

MBS721319

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS721319

products type :

ELISA Kit

products full name :

Human CD30 Ligand ELISA Kit

products short name :

CD30 Ligand

other names :

CD30L protein; Tumor necrosis factor ligand superfamily member 8; tumor necrosis factor ligand superfamily member 8; CD30-L; CD30 ligand; CD153 antigen; CD30 antigen ligand; tumor necrosis factor (ligand) superfamily, member 8; CD30 ligand; CD30-L; CD_antigen: CD153

products gene name :

CD30L

other gene names :

TNFSF8; TNFSF8; CD153; CD30L; CD30LG; CD30L; CD30LG; CD30-L

uniprot entry name :

TNFL8_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CD30L. No significant cross-reactivity or interference between CD30L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD30L and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This CD30L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD30L. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: CD30L ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD30L. Standards or samples are then added to the microtiter plate wells and CD30L if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD30L present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD30L are added to each well to "sandwich" the CD30L immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD30L and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD30L concentration in each sample is interpolated from this standard curve.

ncbi gi num :

2815515

ncbi acc num :

AAB97877.1

ncbi mol weight :

26,017 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family. Protein type: Membrane protein, integral; Cytokine; Apoptosis. Chromosomal Location of Human Ortholog: 9q33. Cellular Component: extracellular space; integral to plasma membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: cell proliferation; CD8-positive, alpha-beta T cell differentiation; defense response to Gram-positive bacterium; cell-cell signaling; apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; signal transduction

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage