ELISA/assay

Human C type lectin domain family 2 member D (CLEC2D) ELISA Kit

MBS7213167

48-Strip-Wells

440 USD

ELISA Kit

Human C type lectin domain family 2 member D (CLEC2D) ELISA Kit

[C type lectin domain family 2 member D (CLEC2D)]

[C-type lectin domain family 2 member D; C-type lectin domain family 2 member D; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, member D; C-type lectin domain family 2, member D; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin]

[CLEC2D]

[CLEC2D; CLEC2D; CLAX; LLT1; OCIL; CLAX; LLT1; OCIL; LLT-1]

CLC2D_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CLEC2D. No significant cross-reactivity or interference between CLEC2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC2D and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 1.0-25ng/mL. Sensitivity: 0.1ng/mL

Immunology

Intended Uses: This CLEC2D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC2D. Principle of the Assay: CLEC2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC2D antibody and an CLEC2D-HRP conjugate. The assay sample and buffer are incubated together with CLEC2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC2D concentration since CLEC2D from samples and CLEC2D-HRP conjugate compete for the anti-CLEC2D antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC2D from the sample, fewer sites are left to bind CLEC2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC2D concentration in each sample is interpolated from this standard curve.

74753331

Q9UHP7.1

10,823 Da

This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Oct 2010]

CLEC2D: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon- gamma. Binds high molecular weight sulfated glycosaminoglycans. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: cell surface; membrane; endoplasmic reticulum; integral to plasma membrane. Molecular Function: transmembrane receptor activity; carbohydrate binding. Biological Process: cell surface receptor linked signal transduction

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415