catalog number :
MBS721259
products type :
ELISA Kit
products full name :
Human Desmoplakin ELISA Kit
products short name :
Desmoplakin
other names :
desmoplakin; Desmoplakin; desmoplakin; Desmoplakin
other gene names :
desmoplakin; desmoplakin; N/A
uniprot entry name :
Q7T9S0_GVAO
specificity :
This assay has high sensitivity and excellent specificity for detection of DSP. No significant cross-reactivity or interference between DSP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DSP and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This DSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DSP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Sensitivity: The sensitivity in this assay is 0.1 ng/mL.
products categories :
Cell Biology
products description :
Principle of the assay: DSP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DSP. Standards or samples are then added to the microtiter plate wells and DSP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DSP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DSP are added to each well to "sandwich" the DSP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DSP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_872549.1
ncbi gb acc num :
NC_005038.1
ncbi mol weight :
63,196 Da