Human Aspartoacylase (ASPA) ELISA Kit | MyBioSource MBS7212388 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Aspartoacylase (ASPA) ELISA Kit

catalog :

MBS7212388

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7212388

products type :

ELISA Kit

products full name :

Human Aspartoacylase (ASPA) ELISA Kit

products short name :

[Aspartoacylase (ASPA)]

other names :

[aspartoacylase; Aspartoacylase; aspartoacylase; ACY-2; aminoacylase 2; aminoacylase-2; aspartoacylase; Aminoacylase-2; ACY-2]

products gene name :

[ASPA]

other gene names :

[ASPA; ASPA; ASP; ACY2; ACY2; ASP; ACY-2]

uniprot entry name :

ACY2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ASMA. No significant cross-reactivity or interference between ASMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASMA and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ug/mL.

products description :

Intended Uses: This ASMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ASMA ELISA kit applies the competitive enzyme immunoassay technique utilizing Smooth Muscle antigen and an ASMA-HRP conjugate. The assay sample and buffer are incubated together with ASMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASMA concentration since ASMA from samples and ASMA-HRP conjugate compete for the Smooth Muscle antigen binding site. Since the number of sites is limited, as more sites are occupied by ASMA from the sample, fewer sites are left to bind ASMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASMA concentration in each sample is interpolated from this standard curve.

ncbi gi num :

189339202

ncbi acc num :

NP_001121557.1

ncbi gb acc num :

NM_001128085.1

ncbi mol weight :

35,735 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Histidine Metabolism Pathway (82958); Histidine Metabolism Pathway (324); Metabolic Pathways (132956)

ncbi summary :

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ASPA: Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids. Defects in ASPA are the cause of Canavan disease (CAND); also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average. Belongs to the AspA/AstE family. Aspartoacylase subfamily. Protein type: EC 3.5.1.15; Hydrolase; Amino Acid Metabolism - histidine; Amino Acid Metabolism - alanine, aspartate and glutamate. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; metal ion binding; hydrolase activity, acting on ester bonds; aspartoacylase activity; aminoacylase activity. Biological Process: myelination in the central nervous system; aspartate catabolic process; positive regulation of oligodendrocyte differentiation. Disease: Canavan Disease

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage