Human C Type Natriuretic Peptide ELISA Kit | MyBioSource MBS721233 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human C Type Natriuretic Peptide ELISA Kit

catalog :

MBS721233

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS721233

products type :

ELISA Kit

products full name :

Human C Type Natriuretic Peptide ELISA Kit

products short name :

C Type Natriuretic Peptide

other names :

C-type natriuretic peptide; C-type natriuretic peptide; C-type natriuretic peptide; natriuretic peptide precursor C; natriuretic peptide C

products gene name :

CNP

other gene names :

NPPC; NPPC; CNP; CNP2; CNP2

uniprot entry name :

ANFC_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CNP. No significant cross-reactivity or interference between CNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0pg/mL.

products description :

Intended Uses: This CNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CNP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CNP. Standards or samples are then added to the microtiter plate wells and CNP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CNP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CNP are added to each well to “sandwich” the CNP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CNP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

13249346

ncbi acc num :

NP_077720.1

ncbi gb acc num :

NM_024409.2

ncbi mol weight :

13,246 Da

ncbi summary :

The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]

uniprot summary :

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. Protein type: Hormone; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2q37.1. Cellular Component: extracellular space; secretory granule. Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding. Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; positive regulation of vasodilation; negative regulation of DNA metabolic process; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage