Human Asialoglycoprotein Receptor 1 ELISA Kit | MyBioSource MBS721214 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Asialoglycoprotein Receptor 1 ELISA Kit

catalog :

MBS721214

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS721214

products type :

ELISA Kit

products full name :

Human Asialoglycoprotein Receptor 1 ELISA Kit

products short name :

Asialoglycoprotein Receptor 1

other names :

asialoglycoprotein receptor 1 isoform b; Asialoglycoprotein receptor 1; asialoglycoprotein receptor 1; hepatic lectin H1; C-type lectin domain family 4 member H1; asialoglycoprotein receptor 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1

products gene name :

ASGR1

other gene names :

ASGR1; ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1; CLEC4H1; ASGP-R 1; ASGPR 1; HL-1

uniprot entry name :

ASGR1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ASGR1. No significant cross-reactivity or interference between ASGR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASGR1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This ASGR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASGR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ASGR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASGR1. Standards or samples are then added to the microtiter plate wells and ASGR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASGR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASGR1 are added to each well to “sandwich” the ASGR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASGR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASGR1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

308387363

ncbi acc num :

NP_001184145.1

ncbi gb acc num :

NM_001197216.2

ncbi mol weight :

29,144 Da

ncbi pathways :

Thyroid Hormone Synthesis Pathway (835410); Thyroid Hormone Synthesis Pathway (839541)

ncbi summary :

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

uniprot summary :

ASGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: integral to plasma membrane; extracellular region. Molecular Function: protein binding; protein homodimerization activity; metal ion binding; asialoglycoprotein receptor activity; carbohydrate binding. Biological Process: receptor-mediated endocytosis; cellular response to extracellular stimulus

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage