ELISA/assay

Rabbit Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit

MBS721199

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit

Neurotrophic Tyrosine Kinase Receptor Type 2

Tyrosine-protein kinase receptor

NTRK2

NTRK2

Q548C2_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Neurobiology

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This SOX1 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SOX1 in the sample, this SOX1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SOX1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of SOX1 utilizes a multiclonal anti-SOX1 antibody and an SOX1-HRP conjugate. The assay sample and buffer are incubated together with SOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOX1 concentration since SOX1 from samples and SOX1-HRP conjugate compete for the anti-SOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOX1 from the sample, fewer sites are left to bind SOX1-HRP conjugate. Standards of known SOX1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of SOX1. The SOX1 concentration in each sample is interpolated from this standard curve.

55665031

CAH71817.1

Q548C2

91,999 Da

48-Strip-Wells

470 USD

96-Strip-Wells

675