ELISA/assay

Rat Hepcidin Antimicrobial Peptide ELISA Kit

MBS721156

48-Strip-Wells

470 USD

ELISA Kit

Rat Hepcidin Antimicrobial Peptide ELISA Kit

Hepcidin Antimicrobial Peptide

Hepcidin antimicrobial peptide; Hepcidin; hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1; hepcidin antimicrobial peptide; Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor; PLTR

HAMP

HAMP; HAMP; HEPC; PLTR; HFE2B; LEAP1; HEPC; LEAP1; UNQ487/PRO1003; LEAP-1; PLTR; Hepc25; Hepc20

HEPC_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of HAMP. No significant cross-reactivity or interference between HAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HAMP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This HAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: HAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HAMP antibody and an HAMP-HRP conjugate. The assay sample and buffer are incubated together with HAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAMP concentration since HAMP from samples and HAMP-HRP conjugate compete for the anti-HAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by HAMP from the sample, fewer sites are left to bind HAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAMP concentration in each sample is interpolated from this standard curve.

18088845

AAH20612.1

9,408 Da

Iron Metabolism In Placenta Pathway (672461)

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: apical cortex; extracellular region. Molecular Function: hormone activity. Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus. Disease: Hemochromatosis, Type 2b

48-Strip-Wells

470 USD

96-Strip-Wells

675