ELISA/assay

Rat Anti-Histone Antibody ELISA Kit

MBS721149

96 Strip Wells

675 USD

ELISA Kit

Rat Anti-Histone Antibody ELISA Kit

Anti-Histone Antibody

histone; Histone H2A.Z; histone H2A.Z; H2AZ histone; H2A histone family, member Z

AHA

H2AFZ; H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1; H2AZ; H2A/z

H2AZ_HUMAN

Rat

Store all reagents at 2-8 degree C.

Signal Transduction

Intended Uses: This AHA ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AHA in the sample, this AHA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AHA concentration. The concentration of AHA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This AHA enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AHA. Standards or samples are then added to the microtiter plate wells and AHA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AHA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AHA are added to each well to "sandwich" the AHA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AHA and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

3649600

AAC61625.1

13,553 Da

Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Amyloids Pathway 366238!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cellular Senescence Pathway 905991!!Cellular Responses To Stress Pathway 645258!!Chromatin Modifying Enzymes Pathway 941403!!Chromatin Organization Pathway 941402

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD