ELISA/assay

Mouse Cysteine and glycine-rich protein 3 (CSRP3) ELISA Kit

MBS7211399

48-Strip-Wells

440 USD

ELISA Kit

Mouse Cysteine and glycine-rich protein 3 (CSRP3) ELISA Kit

[Cysteine and glycine-rich protein 3 (CSRP3)]

[cysteine and glycine-rich protein 3; Cysteine and glycine-rich protein 3; cysteine and glycine-rich protein 3; LIM domain only 4; cardiac LIM domain protein; muscle lim protein isoform; cysteine and glycine-rich protein 3 (cardiac LIM protein); Cardiac LIM protein; Cysteine-rich protein 3; CRP3; LIM domain protein, cardiac; Muscle LIM protein]

[CSRP3]

[CSRP3; CSRP3; CLP; MLP; CRP3; LMO4; CMD1M; CMH12; CLP; MLP; CRP3]

CSRP3_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CSRP3. No significant cross-reactivity or interference between CSRP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSRP3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Epigenetics and Nuclear Signaling

Intended Uses: This CSRP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCSRP3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CSRP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CSRP3 antibody and an CSRP3-HRP conjugate. The assay sample and buffer are incubated together with CSRP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSRP3 concentration since CSRP3 from samples and CSRP3-HRP conjugate compete for the anti-CSRP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSRP3 from the sample, fewer sites are left to bind CSRP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSRP3 concentration in each sample is interpolated from this standard curve.

Glomerular filtrate proteins in acute cardiorenal syndrome Rumie Wakasaki, Katsuyuki Matsushita, Kirsti Golgotiu,1 Sharon Anderson, Mahaba B. Eiwaz, Daniel J. Orton, Sang Jun Han, H. Thomas Lee, Richard D. Smith, Karin D. Rodland, Paul D. Piehowski, and Michael P. Hutchens.

4502893

NP_003467.1

NM_003476.4

20,969 Da

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

CSRP3: Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in CSRP3 are the cause of familial hypertrophic cardiomyopathy type 12 (CMH12). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Protein type: Transcription regulation. Chromosomal Location of Human Ortholog: 11p15.1. Cellular Component: cytoskeleton; Z disc; nucleus. Molecular Function: actinin binding; protein binding; telethonin binding; zinc ion binding; structural constituent of muscle. Biological Process: cardiac muscle development; cellular calcium ion homeostasis; skeletal muscle development; protein localization in organelle; cardiac myofibril assembly; positive regulation of transcription from RNA polymerase II promoter; regulation of the force of heart contraction; cardiac muscle contraction. Disease: Cardiomyopathy, Familial Hypertrophic, 12; Cardiomyopathy, Dilated, 1m

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415