ELISA/assay

Rat Protein Kinase C Iota ELISA Kit

MBS721116

48-Strip-Wells

470 USD

ELISA Kit

Rat Protein Kinase C Iota ELISA Kit

Protein Kinase C Iota

protein kinase C iota; Protein kinase C iota type; protein kinase C iota type; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; protein kinase C, iota; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota

PKCi

PRKCI; PRKCI; PKCI; DXS1179E; nPKC-iota; DXS1179E; PRKC-lambda/iota; aPKC-lambda/iota

KPCI_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This PKCI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PKCI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PKCI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PKCI. Standards or samples are then added to the microtiter plate wells and PKCI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PKCI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PKCI are added to each well to "sandwich" the PKCI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PKCI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCI concentration in each sample is interpolated from this standard curve.

598225

AAB17011.1

68,262 Da

Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Corticotropin-releasing Hormone Pathway (920957); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279); Endocytosis Pathway (102181); G Protein Signaling Pathways (198849); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Protein type: Tumor suppressor; Cell adhesion; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Protein kinase, AGC; EC 2.7.11.13; Motility/polarity/chemotaxis; Cell development/differentiation; AGC group; PKC family; Iota subfamily. Chromosomal Location of Human Ortholog: 3q26.3. Cellular Component: Golgi membrane; microtubule cytoskeleton; intercellular bridge; protein complex; tight junction; apical plasma membrane; cytoplasm; leading edge; plasma membrane; nucleus; cytosol; endosome. Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; metal ion binding; phospholipid binding; ATP binding; protein kinase activity. Biological Process: intercellular junction assembly and maintenance; cell migration; secretion; nerve growth factor receptor signaling pathway; establishment of apical/basal cell polarity; actin filament organization; cytoskeleton organization and biogenesis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; vesicle-mediated transport; establishment and/or maintenance of epithelial cell polarity; positive regulation of glucose import; eye photoreceptor cell development; cellular response to insulin stimulus; Golgi vesicle budding; negative regulation of neuron apoptosis; protein targeting to membrane; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675