ELISA/assay

Canine Interleukin-4 receptor subunit alpha (IL4R) ELISA Kit

MBS7211033

48-Strip-Wells

440 USD

ELISA Kit

Canine Interleukin-4 receptor subunit alpha (IL4R) ELISA Kit

[Interleukin-4 receptor subunit alpha (IL4R)]

[interleukin-4 receptor subunit alpha isoform a; Interleukin-4 receptor subunit alpha; interleukin-4 receptor subunit alpha; IL4R nirs variant 1; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; interleukin 4 receptor; CD_antigen: CD124Cleaved into the following chain:Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/protAlternative name(s):IL-4-binding protein; IL4-BP]

[IL4R]

[IL4R; IL4R; CD124; IL4RA; IL-4RA; IL4RA; 582J2.1; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP]

IL4RA_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of IL-4. No significant cross-reactivity or interference between IL-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This IL-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-4 antibody and an IL-4-HRP conjugate. The assay sample and buffer are incubated together with IL-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-4 concentration since IL-4 from samples and IL-4-HRP conjugate compete for the anti-IL-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-4 from the sample, fewer sites are left to bind IL-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-4 concentration in each sample is interpolated from this standard curve.

4557669

NP_000409.1

NM_000418.3

88,267 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL-4 Signaling Pathway (198760); IL4-mediated Signaling Events Pathway (137933); Inflammatory Response Pathway (198766); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077)

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16p12.1-p11.2. Cellular Component: extracellular space; integral to plasma membrane; receptor complex. Molecular Function: protein binding; interleukin-4 receptor activity; receptor signaling protein activity. Biological Process: ovulation; positive regulation of T-helper 2 cell differentiation; negative regulation of T-helper 1 cell differentiation; production of molecular mediator of acute inflammatory response; response to estrogen stimulus; immune response; defense response to protozoan; signal transduction; regulation of cell proliferation; positive regulation of macrophage activation. Disease: Ige Responsiveness, Atopic; Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415