ELISA/assay

Human Enkephalin ELISA Kit

MBS721102

48-Strip-Wells

470 USD

ELISA Kit

Human Enkephalin ELISA Kit

Enkephalin

enkephalin; Proenkephalin-A; proenkephalin-A; proenkephalin 1; proenkephalin 2; preproenkephalin 2; proenkephalin related sequence; proenkephalin; Opioid growth factor; OGF

ENK

Penk; Penk; Enk; Penk1; Penk2; Penk-rs; Penk-rs; Penk1; OGF

PENK_RAT

Human

This assay has high sensitivity and excellent specificity for detection of ENK. No significant cross-reactivity or interference between ENK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ENK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This ENK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ENK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Princple of the assay: ENK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ENK. Standards or samples are then added to the microtiter plate wells and ENK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ENK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ENK are added to each well to “sandwich” the ENK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ENK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENK concentration in each sample is interpolated from this standard curve.

204026

AAA41115.1

30,932 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (i) Signalling Events Pathway (1010195); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Peptide Ligand-binding Receptors Pathway (1010165); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)

neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006]

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family. Protein type: Hormone; Secreted; Secreted, signal peptide

48-Strip-Wells

470 USD

96-Strip-Wells

675