ELISA/assay

Rabbit Cathepsin S ELISA Kit

MBS721067

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Cathepsin S ELISA Kit

Cathepsin S

cathepsin S; Cathepsin S; cathepsin S; cathepsin S

Cath-S

CTSS; CTSS

CATS_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This CTSS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CTSS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY CTSS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTSS antibody and an CTSS-HRP conjugate. The assay sample and buffer are incubated together with CTSS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSS concentration since CTSS from samples and CTSS-HRP conjugate compete for the anti-CTSS antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSS from the sample, fewer sites are left to bind CTSS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSS concentration in each sample is interpolated from this standard curve.

179957

AAC37592.1

31,626 Da

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Endosomal/Vacuolar Pathway (477125); Extracellular Matrix Organization Pathway (576262)

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.22.27; Protease. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: lysosomal lumen; extracellular space; membrane; intracellular membrane-bound organelle; lysosome; extracellular region. Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding. Biological Process: extracellular matrix organization and biogenesis; adaptive immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; extracellular matrix disassembly; collagen catabolic process; antigen processing and presentation; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675