ELISA/assay

Mouse Matrix extracellular phosphoglycoprotein ELISA Kit

MBS721044

48-Strip-Wells

470 USD

ELISA Kit

Mouse Matrix extracellular phosphoglycoprotein ELISA Kit

Matrix extracellular phosphoglycoprotein

Matrix extracellular phosphoglycoprotein; Matrix extracellular phosphoglycoprotein; matrix extracellular phosphoglycoprotein; osteoblast/osteocyte factor 45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; Osteoblast/osteocyte factor 45; OF45

MEPE

MEPE; MEPE; OF45; OF45

MEPE_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This MEPE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MEPE. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This MEPE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MEPE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the Assay: MEPE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MEPE antibody and an MEPE-HRP conjugate. The assay sample and buffer are incubated together with MEPE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MEPE concentration since MEPE from samples and MEPE-HRP conjugate compete for the anti-MEPE antibody binding site. Since the number of sites is limited, as more sites are occupied by MEPE from the sample, fewer sites are left to bind MEPE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MEPE concentration in each sample is interpolated from this standard curve.

118764291

AAI28159.1

45,291 Da

This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

MEPE: Promotes renal phosphate excretion and modulates mineralization. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: proteinaceous extracellular matrix. Molecular Function: protein binding; extracellular matrix structural constituent. Biological Process: negative regulation of bone mineralization; biomineral formation; regulation of bone remodeling; skeletal development

48-Strip-Wells

470 USD

96-Strip-Wells

675