Porcine Acyl-CoA desaturase (SCD) ELISA Kit | MyBioSource MBS7210090 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Acyl-CoA desaturase (SCD) ELISA Kit

catalog :

MBS7210090

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7210090

products type :

ELISA Kit

products full name :

Porcine Acyl-CoA desaturase (SCD) ELISA Kit

products short name :

[Acyl-CoA desaturase (SCD)]

other names :

[acyl-CoA desaturase; Acyl-CoA desaturase; acyl-CoA desaturase; delta-9 desaturase; delta-9-desaturase; delta(9)-desaturase; fatty acid desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase opposite strand; stearoyl-CoA desaturase (delta-9-desaturase); Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase; Stearoyl-CoA desaturase]

products gene name :

[SCD]

other gene names :

[SCD; SCD; SCD1; FADS5; SCDOS; MSTP008; Delta-9 desaturase]

uniprot entry name :

ACOD_HUMAN

reactivity :

Porcine

specificity :

This assay has high sensitivity and excellent specificity for detection of SCD. No significant cross-reactivity or interference between SCD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCD and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products description :

Intended Uses: This SCD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SCD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SCD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCD antibody and an SCD-HRP conjugate. The assay sample and buffer are incubated together with SCD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCD concentration since SCD from samples and SCD-HRP conjugate compete for the anti-SCD antibody binding site. Since the number of sites is limited, as more sites are occupied by SCD from the sample, fewer sites are left to bind SCD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCD concentration in each sample is interpolated from this standard curve.

ncbi gi num :

53759151

ncbi acc num :

NP_005054.3

ncbi gb acc num :

NM_005063.4

ncbi mol weight :

41,523 Da

ncbi pathways :

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Biosynthesis Of Unsaturated Fatty Acids Pathway (83034); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acid Biosynthesis Pathway (198873); Fatty Acid Metabolism Pathway (868084); Fatty Acid Metabolism Pathway (878045); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Feb 2012]

uniprot summary :

SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family. Protein type: EC 1.14.19.1; Endoplasmic reticulum; Oxidoreductase; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 10q24.31. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane. Molecular Function: iron ion binding; stearoyl-CoA 9-desaturase activity. Biological Process: fatty acid biosynthetic process

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage