ELISA/assay

Human Medium wave sensitive opsin 1 (OPN1MW) ELISA Kit

MBS7209879

48-Strip-Wells

470 USD

ELISA Kit

Human Medium wave sensitive opsin 1 (OPN1MW) ELISA Kit

Medium wave sensitive opsin 1 (OPN1MW)

medium-wave-sensitive opsin 1; Medium-wave-sensitive opsin 1; medium-wave-sensitive opsin 1; green cone pigment; green-sensitive opsin; photopigment apoprotein; cone dystrophy 5 (X-linked); green cone photoreceptor pigment; opsin 1 (cone pigments), medium-wave-sensitive; Green cone photoreceptor pigment; Green-sensitive opsin; GOP

OPN1MW

OPN1MW; OPN1MW; CBD; GCP; GOP; CBBM; COD5; OPN1MW1; GCP OPN1MW2 ; GOP

OPSG_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of OPN1MW. No significant cross-reactivity or interference between OPN1MW and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPN1MW and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This OPN1MW ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OPN1MW. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: OPN1MW ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPN1MW antibody and an OPN1MW-HRP conjugate. The assay sample and buffer are incubated together with OPN1MW-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPN1MW concentration since OPN1MW from samples and OPN1MW-HRP conjugate compete for the anti-OPN1MW antibody binding site. Since the number of sites is limited, as more sites are occupied by OPN1MW from the sample, fewer sites are left to bind OPN1MW-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPN1MW concentration in each sample is interpolated from this standard curve.

4503965

NP_000504.1

NM_000513.2

40,584 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Opsins Pathway (106377); Retinoid Cycle Disease Events Pathway (771582); Signal Transduction Pathway (477114)

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness. [provided by RefSeq, Mar 2009]

OPN1MW: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Defects in OPN1MW are the cause of partial colorblindness deutan series (CBD); also known as deuteranopia. Defects in OPN1MW are a cause of blue cone monochromacy (BCM). A rare X-linked congenital stationary cone dysfunction syndrome characterized by the absence of functional long wavelength-sensitive and medium wavelength-sensitive cones in the retina. Color discrimination is severely impaired from birth, and vision is derived from the remaining preserved blue (S) cones and rod photoreceptors. BCM typically presents with reduced visual acuity, pendular nystagmus, and photophobia. Patients often have myopia. Defects in OPN1MW are a cause of cone dystrophy type 5 (COD5). A X-linked cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; photoreceptor activity. Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; phototransduction, visible light; visual perception; retinoid metabolic process; protein-chromophore linkage. Disease: Blue Cone Monochromacy; Colorblindness, Partial, Deutan Series

48-Strip-Wells

470 USD

96-Strip-Wells

675