catalog number :
MBS720972
products type :
ELISA Kit
products full name :
Mouse Ovarian cancer marker/Carbohydrate antigen 125 ELISA Kit
products short name :
Ovarian cancer marker/Carbohydrate antigen 125
products gene name :
Ovarian cancer marker-CA125
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This CA125 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CA125. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Sensitivity: The sensitivity in this assay is 0.1 ng/mL.
products categories :
Cancer
products description :
Principle of the Assay: CA125 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA125 antibody and an CA125-HRP conjugate. The assay sample and buffer are incubated together with CA125-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA125 concentration since CA125 from samples and CA125-HRP conjugate compete for the anti-CA125 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA125 from the sample, fewer sites are left to bind CA125-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA125 concentration in each sample is interpolated from this standard curve.