catalog number :
MBS720969
products type :
ELISA Kit
products full name :
Human Tyrosinase ELISA Kit
products short name :
Tyrosinase
other names :
TYR, partial; TYR; TYR
uniprot entry name :
F5CQG8_MEGNO
specificity :
This assay has high sensitivity and excellent specificity for detection of TYR. No significant cross-reactivity or interference between TYR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TYR and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.
products description :
Intended Uses: This TYR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TYR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TYR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TYR antibody and an TYR-HRP conjugate. The assay sample and buffer are incubated together with TYR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TYR concentration since TYR from samples and TYR-HRP conjugate compete for the anti-TYR antibody binding site. Since the number of sites is limited, as more sites are occupied by TYR from the sample, fewer sites are left to bind TYR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TYR concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AEB71713.1
ncbi mol weight :
19,455 Da