ELISA/assay

Canine Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

MBS7209551

48-Strip-Wells

440 USD

ELISA Kit

Canine Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

[Troponin I, fast skeletal muscle (TNNI2)]

[troponin I, fast skeletal muscle; Troponin I, fast skeletal muscle; troponin I, fast skeletal muscle; troponin I, fast-twitch isoform; Troponin I, fast-twitch isoform]

[TNNI2]

[TNNI2; TNNI2; TnI]

TNNI2_RABIT

Canine

This assay has high sensitivity and excellent specificity for detection of TNNI1. No significant cross-reactivity or interference between TNNI1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TNNI1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.

Cardiovascular

Intended Uses: This TNNI1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TNNI1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TNNI1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TNNI1 antibody and an TNNI1-HRP conjugate. The assay sample and buffer are incubated together with TNNI1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNNI1 concentration since TNNI1 from samples and TNNI1-HRP conjugate compete for the anti-TNNI1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TNNI1 from the sample, fewer sites are left to bind TNNI1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNNI1 concentration in each sample is interpolated from this standard curve.

130493079

NP_001076252.1

NM_001082783.1

21,214 Da

TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415