Rat Prostaglandin E Synthase, Microsomal ELISA Kit | MyBioSource MBS720955 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Prostaglandin E Synthase, Microsomal ELISA Kit

catalog :

MBS720955

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720955

products type :

ELISA Kit

products full name :

Rat Prostaglandin E Synthase, Microsomal ELISA Kit

products short name :

Prostaglandin E Synthase, Microsomal

other names :

prostaglandin E synthase; Prostaglandin E synthase; prostaglandin E synthase; MGST1-like 1; p53-induced gene 12 protein; p53-induced apoptosis protein 12; glutathione S-transferase 1-like 1; microsomal prostaglandin E synthase 1; microsomal prostaglandin E synthase-1; tumor protein p53 inducible protein 12; microsomal glutathione S-transferase 1-like 1; prostaglandin E synthase; Microsomal glutathione S-transferase 1-like 1; MGST1-L1; Microsomal prostaglandin E synthase 1; MPGES-1; p53-induced gene 12 protein

products gene name :

PTGES

other gene names :

PTGES; PTGES; PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1; MGST1L1; MPGES1; PGES; PIG12; MGST1-L1; MPGES-1

uniprot entry name :

PTGES_HUMAN

reactivity :

Rat

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

products description :

Intended Uses: This PGEsm ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PGEsm. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PGEsm ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PGEsm. Standards or samples are then added to the microtiter plate wells and PGEsm if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PGEsm present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PGEsm are added to each well to "sandwich" the PGEsm immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PGEsm and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGEsm concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4758910

ncbi acc num :

NP_004869.1

ncbi gb acc num :

NM_004878.4

ncbi mol weight :

17,102 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Synthesis Of Prostaglandins (PG) And Thromboxanes (TX) Pathway (730333)

ncbi summary :

The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]

uniprot summary :

PTGES: Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Belongs to the MAPEG family. Protein type: Membrane protein, multi-pass; EC 5.3.99.3; Nuclear envelope; Lipid Metabolism - arachidonic acid; Membrane protein, integral; Isomerase. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: endoplasmic reticulum membrane; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; integral to membrane. Molecular Function: glutathione binding; prostaglandin-E synthase activity. Biological Process: negative regulation of cell proliferation; chronic inflammatory response; response to retinoic acid; response to cytokine stimulus; cyclooxygenase pathway; arachidonic acid metabolic process; response to lipopolysaccharide; response to calcium ion; prostaglandin biosynthetic process; signal transduction; response to organic cyclic substance; acute inflammatory response; prostaglandin metabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage