Human Autophagy protein 5 (ATG5) ELISA Kit | MyBioSource MBS7209535 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Autophagy protein 5 (ATG5) ELISA Kit

catalog :

MBS7209535

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7209535

products type :

ELISA Kit

products full name :

Human Autophagy protein 5 (ATG5) ELISA Kit

products short name :

[Autophagy protein 5 (ATG5)]

other names :

[autophagy protein 5; Autophagy protein 5; autophagy protein 5; ATG5 autophagy related 5 homolog; autophagy related 5]

products gene name :

[ATG5]

other gene names :

[Atg5; Atg5]

uniprot entry name :

ATG5_RAT

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ATF5. No significant cross-reactivity or interference between ATF5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF5 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products description :

Intended Uses: This ATF5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ATF5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATF5 antibody and an ATF5-HRP conjugate. The assay sample and buffer are incubated together with ATF5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF5 concentration since ATF5 from samples and ATF5-HRP conjugate compete for the anti-ATF5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF5 from the sample, fewer sites are left to bind ATF5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF5 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

62079231

ncbi acc num :

NP_001014272.1

ncbi gb acc num :

NM_001014250.1

ncbi mol weight :

32,398 Da

ncbi pathways :

RIG-I-like Receptor Signaling Pathway (117250); RIG-I-like Receptor Signaling Pathway (116128); Regulation Of Autophagy Pathway (83450); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (219773)

uniprot summary :

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Ubiquitin conjugating system; Autophagy. Cellular Component: protein complex; membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme. Molecular Function: protein binding; APG8 conjugating enzyme activity. Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; negative regulation of protein ubiquitination; blood vessel remodeling; otolith development; autophagic vacuole formation; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage