Rabbit Galectin 3 ELISA Kit | MyBioSource MBS720938 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Galectin 3 ELISA Kit

catalog :

MBS720938

quantity :

48-Strip-Wells-(Competitive)

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS720938

products type :

ELISA Kit

products full name :

Rabbit Galectin 3 ELISA Kit

products short name :

[Galectin 3]

other names :

[galectin-3 isoform 2; Galectin-3; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen]

products gene name :

[GAL3]

other gene names :

[LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP]

uniprot entry name :

LEG3_HUMAN

reactivity :

Rabbit

specificity :

This assay has high sensitivity and excellent specificity for detection of GAL3. No significant cross-reactivity or interference between GAL3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAL3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8°C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Cell Biology

products description :

Intended Uses: This GAL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GAL3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GAL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GAL3 antibody and an GAL3-HRP conjugate. The assay sample and buffer are incubated together with GAL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL3 concentration since GAL3 from samples and GAL3-HRP conjugate compete for the anti-GAL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL3 from the sample, fewer sites are left to bind GAL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

294345475

ncbi acc num :

NP_001170859.1

ncbi gb acc num :

NM_001177388.1

ncbi pathways :

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

uniprot summary :

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages. Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Cell surface. Chromosomal Location of Human Ortholog: 14q22.3. Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane. Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

size1 :

48-Strip-Wells-(Competitive)

price1 :

440 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

440

size3 :

96-Strip-Wells-(Competitive)

price3 :

640

size4 :

96-Strip-Wells-(Sandwich)

price4 :

640

size5 :

5x96-Strip-Wells-(Competitive)

price5 :

2895

size6 :

5x96-Strip-Wells-(Sandwich)

price6 :

2895

size7 :

10x96-Strip-Wells-(Competitive)

price7 :

5415

size8 :

10x96-Strip-Wells-(Sandwich)

price8 :

5415

more info or order :

MyBioSource product webpage