Rat Interleukin 33 ELISA Kit | MyBioSource MBS720902 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Interleukin 33 ELISA Kit

catalog :

MBS720902

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720902

products type :

ELISA Kit

products full name :

Rat Interleukin 33 ELISA Kit

products short name :

Interleukin 33

products name syn :

Rat Interleukin 33 ELISA Kit; Rat Interleukin 33 ELISA Kit; Interleukin 33; Interleukin 33 ELISA Kit (Rat)

other names :

interleukin-33 isoform 3; Interleukin-33; interleukin-33; IL-33; IL-1F11; OTTHUMP00000021041; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules; interleukin 33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules

products gene name :

IL-33

other gene names :

IL33; IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2; C9orf26; IL1F11; NFHEV

uniprot entry name :

IL33_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-33. No significant cross-reactivity or interference between IL-33 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-33 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IL-33 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IL-33. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Rat ELISA Kit

products description :

. Principle of the assay: IL-33 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-33. Standards or samples are then added to the microtiter plate wells and IL-33 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-33 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-33 are added to each well to "sandwich" the IL-33 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-33 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-33 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

314122337

ncbi acc num :

NP_001186570.1

ncbi gb acc num :

NM_001199641.1

uniprot acc num :

O95760

ncbi mol weight :

30,759 Da

ncbi pathways :

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217173); Influenza A Pathway (217150)

ncbi summary :

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM]

uniprot summary :

Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines. Ref.3. Subcellular location: Secreted Ref.1 Ref.3. Tissue specificity: Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. Post-translational modification: Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Sequence similarities: Belongs to the IL-1 family. Highly divergent. Caution: Was originally (Ref.1) thought to one of the key factors that controls the specialized post-capillary high endothelial venules (HEV) phenotype found in organized secondary lymphoid tissue and to be nuclear localized.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage