ELISA/assay

Human Ghrelin ELISA Kit

MBS720885

48-Strip-Wells

470 USD

ELISA Kit

Human Ghrelin ELISA Kit

Ghrelin

ghrelin; Ghrelin; Ghrelin

Ghrelin

E7CCH4_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GHRL. No significant cross-reactivity or interference between GHRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GHRL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0 ng/mL.

Intended Uses: This GHRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GHRL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GHRL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GHRL. Standards or samples are then added to the microtiter plate wells and GHRL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GHRL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GHRL are added to each well to “sandwich” the GHRL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GHRL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHRL concentration in each sample is interpolated from this standard curve.

304435649

ADM33790.1

12,992 Da

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach. Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted; Cell cycle regulation; Apoptosis. Chromosomal Location of Human Ortholog: 3p26-p25. Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity. Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; G-protein coupled receptor protein signaling pathway; positive regulation of synaptogenesis; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; cartilage development; actin polymerization and/or depolymerization; negative regulation of inflammatory response; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis. Disease: Obesity

48-Strip-Wells

470 USD

96-Strip-Wells

675