ELISA/assay

Rat Insulin Like Growth Factor Binding Protein 2 ELISA Kit

MBS720878

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Insulin Like Growth Factor Binding Protein 2 ELISA Kit

Insulin Like Growth Factor Binding Protein 2

Insulin-like growth factor-binding protein 2; Insulin-like growth factor-binding protein 2; insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2; insulin-like growth factor binding protein 2, 36kDa

IGFBP2

IGFBP2; IGFBP2; IBP2; IGF-BP53; BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2

IBP2_HUMAN

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Highest Standards: 250ng/mL. Concentration of Standards: Concentration of Standards: 0,10,25,50,100,250ng/mL

Cell Biology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This IGFBP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IGFBP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY IGFBP-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IGFBP-2. Standards or samples are then added to the microtiter plate wells and IGFBP-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IGFBP-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IGFBP-2 are added to each well to “sandwich” the IGFBP-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGFBP-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP-2 concentration in each sample is interpolated from this standard curve.

290457647

P18065.2

34,814 Da

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle. Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding. Biological Process: response to drug; response to retinoic acid; regulation of insulin-like growth factor receptor signaling pathway; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; signal transduction; positive regulation of activated T cell proliferation; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675