Rat heparin-binding epidermal growth factor-like growth factor ELISA Kit | MyBioSource MBS720867 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat heparin-binding epidermal growth factor-like growth factor ELISA Kit

catalog :

MBS720867

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720867

products type :

ELISA Kit

products full name :

Rat heparin-binding epidermal growth factor-like growth factor ELISA Kit

products short name :

heparin-binding epidermal growth factor-like growth factor

products name syn :

Rat heparin-binding epidermal growth factor-like growth factor ELISA Kit; heparin-binding epidermal growth factor-like growth factor; heparin-binding epidermal growth factor-like growth factor (Rat)

other names :

heparin-binding EGF-like growth factor; Proheparin-binding EGF-like growth factor; proheparin-binding EGF-like growth factor; heparin-binding EGF-like growth factor

products gene name :

HB-EGF

other gene names :

HBEGF; HBEGF; HB-EGF; HEGFL

uniprot entry name :

HBEGF_CHICK

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of HB-EGF-GF. No significant cross-reactivity or interference between HB-EGF-GF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HB-EGF-GF and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This HB-EGF-GF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HB-EGF-GF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Rat ELISA Kit

products description :

Principle of the assay: HB-EGF-GF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HB-EGF-GF. Standards or samples are then added to the microtiter plate wells and HB-EGF-GF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HB-EGF-GF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HB-EGF-GF are added to each well to “sandwich” the HB-EGF-GF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HB-EGF-GF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HB-EGF-GF concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4761593

uniprot acc num :

Q9W7C5

ncbi mol weight :

22,541 Da

ncbi pathways :

ErbB Signaling Pathway (83957); ErbB Signaling Pathway (458); GnRH Signaling Pathway (119321); GnRH Signaling Pathway (502)

uniprot summary :

Function: May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. By similarity. Plays an important role in the proper development of cranial nerves by inhibting the migration of the cranial neural crest cells (NCCs) migration into the odd-numbered neuromeres (r3 and r5) of the hindbrain. Plays a role in mediating v-Jun-induced oncogenic transformation. Ref.1 Ref.2. Subunit structure: Interacts with CNIH2. Ref.2. Subcellular location: Heparin-binding EGF-like growth factor: Secreted extracellular space. By similarity. Note: Mature HB-EGF is released into the extracellular space and probably binds to a receptor. By similarity.Proheparin-binding EGF-like growth factor: Cell membrane; Single-pass type I membrane protein. Developmental stage: Expressed uniformly in the embryonic hindbrain. Ref.2. Induction: Strongly induced in embryonic fibroblasts transformed by v-Jun. Ref.1. Sequence similarities: Contains 1 EGF-like domain.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage