ELISA/assay

Rabbit Recombination activating gene 2 ELISA Kit

MBS720824

96 Strip Wells

675 USD

ELISA Kit

Rabbit Recombination activating gene 2 ELISA Kit

Recombination activating gene 2

pregnancy-associated glycoprotein 2; Pregnancy-associated glycoprotein 2; pregnancy-associated glycoprotein 2; PAG 2; aspartic proteinase; pregnancy-associated glycoprotein 10

RAG-2

PAG6; PAG2; PAG2; PAG10; PAG 2

PAG2_PIG

Rabbit

Store all reagents at 2-8 degree C.

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This RAG2 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of RAG2 in the sample, this RAG2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus RAG2 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of RAG2 utilizes a multiclonal anti-RAG2 antibody and an RAG2-HRP conjugate. The assay sample and buffer are incubated together with RAG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAG2 concentration since RAG2 from samples and RAG2-HRP conjugate compete for the anti-RAG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAG2 from the sample, fewer sites are left to bind RAG2-HRP conjugate. Standards of known RAG2 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of RAG2. The RAG2 concentration in each sample is interpolated from this standard curve.

55741443

NP_001001536.2

NM_001001536.2

47,132 Da

Protein Digestion And Absorption Pathway 172825!!Protein Digestion And Absorption Pathway 171868

96 Strip Wells

675 USD