Rabbit Interleukin 1 ELISA Kit | MyBioSource MBS720797 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Interleukin 1 ELISA Kit

catalog :

MBS720797

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720797

products type :

ELISA Kit

products full name :

Rabbit Interleukin 1 ELISA Kit

products short name :

Interleukin 1

other names :

interleukin-1 alpha proprotein; Interleukin-1 alpha; interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha; interleukin 1, alpha; Hematopoietin-1

products gene name :

IL-1

other gene names :

IL1A; IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL1F1; IL-1 alpha

uniprot entry name :

IL1A_HUMAN

reactivity :

Rabbit

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-1. No significant cross-reactivity or interference between IL-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: IL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-1 antibody and an IL-1-HRP conjugate. The assay sample and buffer are incubated together with IL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1 concentration since IL-1 from samples and IL-1-HRP conjugate compete for the anti-IL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1 from the sample, fewer sites are left to bind IL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

27894330

ncbi acc num :

NP_000566.3

ncbi gb acc num :

NM_000575.3

ncbi mol weight :

30,607 Da

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); FAS Pathway And Stress Induction Of HSP Regulation (198894); Graft-versus-host Disease Pathway (83124)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Protein type: Motility/polarity/chemotaxis; Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; positive regulation of angiogenesis; connective tissue replacement during inflammatory response; response to copper ion; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of cytokine secretion

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage