ELISA/assay

Mouse Probable ATP-dependent RNA helicase DDX58 (DDX58) ELISA Kit

MBS7207932

48-Strip-Wells

470 USD

ELISA Kit

Mouse Probable ATP-dependent RNA helicase DDX58 (DDX58) ELISA Kit

Probable ATP-dependent RNA helicase DDX58 (DDX58)

probable ATP-dependent RNA helicase DDX58; Probable ATP-dependent RNA helicase DDX58; probable ATP-dependent RNA helicase DDX58; RIG-1; RNA helicase RIG-I; DEAD box protein 58; RIG-I-like receptor 1; retinoic acid inducible gene I; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; RIG-I-like receptor 1; RLR-1; Retinoic acid-inducible gene 1 protein; RIG-1; Retinoic acid-inducible gene I protein; RIG-I

DDX58

DDX58; DDX58; RIGI; RIG-I; RLR-1; RLR-1; RIG-1; RIG-I

DDX58_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of DDX58. No significant cross-reactivity or interference between DDX58 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDX58 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This DDX58 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DDX58. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: DDX58 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDX58 antibody and an DDX58-HRP conjugate. The assay sample and buffer are incubated together with DDX58-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX58 concentration since DDX58 from samples and DDX58-HRP conjugate compete for the anti-DDX58 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX58 from the sample, fewer sites are left to bind DDX58-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX58 concentration in each sample is interpolated from this standard curve.

27881482

NP_055129.2

NM_014314.3

101,377 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis B Pathway (694606); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]

DDX58: an innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5 - triphosphorylated ssRNA and dsRNA and short dsRNA ( 1 kb in length). In addition to the 5 -triphosphate moiety, blunt-end base pairing at the 5 -end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3 overhang at the 5 triphosphate end decreases and any 5 overhang at the 5 triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein MAVS which activates the IKK- related kinases TBK1 and IKBKE which phosphorylate interferon regulatory factors IRF3 and IRF7, in turn activating transcription of antiviral immunological genes, including IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory synctial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. Maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58, IKBKE, TBK1 and STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58. Interacts with SRC. Interacts with protein Z of Guanarito virus, Machupo virus, Junin arenavirus and Sabia virus. This interaction disrupts its interaction with MAVS, impeding downstream IRF3 and NF-kappa-B activation and resulting in decreased IFN-beta induction. Interacts (via CARD domain) with Human respiratory syncytial virus A non-structural protein 2 (NS2) and this interaction disrupts its interaction with MAVS, impeding downstream IRF3 activation. Present in vascular smooth cells. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.6.4.13. Chromosomal Location of Human Ortholog: 9p12. Cellular Component: tight junction; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: identical protein binding; protein binding; single-stranded RNA binding; zinc ion binding; double-stranded RNA binding; double-stranded DNA binding; helicase activity; ATP binding. Biological Process: viral reproduction; metabolic process; response to virus; regulation of cell migration; response to exogenous dsRNA; detection of virus; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of defense response to virus by host; negative regulation of interferon type I production; positive regulation of interferon-alpha production. Disease: Singleton-merten Syndrome 2

48-Strip-Wells

470 USD

96-Strip-Wells

675