Human Zyxin ELISA Kit | MyBioSource MBS7207836 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Zyxin ELISA Kit

catalog :

MBS7207836

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7207836

products type :

ELISA Kit

products full name :

Human Zyxin ELISA Kit

products short name :

Zyxin

other names :

zyxin; Zyxin; zyxin; zyxin-2; zyxin; Zyxin-2

products gene name :

ZYX

other gene names :

ZYX; ZYX; ESP-2; HED-2

uniprot entry name :

ZYX_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ZYX. No significant cross-reactivity or interference between ZYX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ZYX and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ZYX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZYX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Epigenetics and Nuclear Signaling

products description :

Principle of the assay: ZYX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZYX antibody and an ZYX-HRP conjugate. The assay sample and buffer are incubated together with ZYX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZYX concentration since ZYX from samples and ZYX-HRP conjugate compete for the anti-ZYX antibody binding site. Since the number of sites is limited, as more sites are occupied by ZYX from the sample, fewer sites are left to bind ZYX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZYX concentration in each sample is interpolated from this standard curve.

ncbi gi num :

58530845

ncbi acc num :

NP_001010972.1

ncbi gb acc num :

NM_001010972.1

ncbi mol weight :

61,277 Da

ncbi pathways :

E-cadherin Signaling In Keratinocytes Pathway (138002); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin-mediated Cell Adhesion Pathway (198816); Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway (137918)

ncbi summary :

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

uniprot summary :

Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family. Protein type: Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q32. Cellular Component: cell-cell adherens junction; focal adhesion; integral to plasma membrane; cytoplasm; stress fiber; plasma membrane; nucleus. Molecular Function: protein binding; zinc ion binding. Biological Process: integrin-mediated signaling pathway; cell-cell signaling; viral reproduction; cell-matrix adhesion; transforming growth factor beta receptor signaling pathway; regulation of inflammatory response; stress fiber formation; signal transduction; cell adhesion

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage