catalog number :
MBS720755
products type :
ELISA Kit
products full name :
Mouse Immunoglobulin A ELISA Kit
products short name :
Immunoglobulin A
other names :
IgA, partial
specificity :
This assay has high sensitivity and excellent specificity for detection of IgA. No significant cross-reactivity or interference between IgA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IgA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mg/ml.
other info2 :
Intended Uses: This IgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IgA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IgA antibody and an IgA-HRP conjugate. The assay sample and buffer are incubated together with IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgA concentration since IgA from samples and IgA-HRP conjugate compete for the anti-IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by IgA from the sample, fewer sites are left to bind IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAA10818.1