Rat Hormone Sensitive Lipase ELISA Kit | MyBioSource MBS720741 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Hormone Sensitive Lipase ELISA Kit

catalog :

MBS720741

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS720741

products type :

ELISA Kit

products full name :

Rat Hormone Sensitive Lipase ELISA Kit

products short name :

Hormone Sensitive Lipase

other names :

hormone-sensitive lipase; Hormone-sensitive lipase; hormone-sensitive lipase; hormone-sensitive lipase testicular isoform; lipase, hormone-sensitive

products gene name :

HSL

other gene names :

LIPE; LIPE; HSL; LHS; AOMS4; HSL

uniprot entry name :

LIPS_HUMAN

reactivity :

Rat

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

other info2 :

Intended Uses: This HSL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HSL. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This HSL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HSL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the Assay: HSL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HSL. Standards or samples are then added to the microtiter plate wells and HSL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HSL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HSL are added to each well to "sandwich" the HSL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSL concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21328446

ncbi acc num :

NP_005348.2

ncbi gb acc num :

NM_005357.3

ncbi mol weight :

84,128 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Fatty Acid Beta Oxidation Pathway (198865); Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway (106121); Insulin Signaling Pathway (198845); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Lipid Digestion, Mobilization, And Transport Pathway (106111)

ncbi summary :

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

uniprot summary :

HSL: hormone sensitive lipase is a lipolytic enzyme of the GDXG family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin. Protein type: EC 3.1.1.79; Lipase. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: lipid particle; caveola; cytosol. Molecular Function: hormone-sensitive lipase activity; triacylglycerol lipase activity; protein binding; protein kinase binding. Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process. Disease: Abdominal Obesity-metabolic Syndrome 4

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage