catalog number :
MBS720739
products type :
ELISA Kit
products full name :
Rabbit Phospholipidase A2 ELISA Kit
products short name :
Phospholipidase A2
other names :
phospholipase A2; Phospholipase A2; phospholipase A2; allergen Api m 1; allergen Api m I; phosphatidylcholine 2-acylhydrolase; phospholipase A2; Allergen Api m I; Phosphatidylcholine 2-acylhydrolase; Allergen: Api m 1
products gene name :
PL-A2
other gene names :
Pla2; bvPLA2; bvPLA2; GB13351; bvPLA2
uniprot entry name :
PA2_APIME
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0pg/mL
other info2 :
Intended Uses: This PLA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PLA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications
products categories :
Cardiovascular
products description :
Principle of the Assay: PLA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLA2 antibody and an PLA2-HRP conjugate. The assay sample and buffer are incubated together with PLA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLA2 concentration since PLA2 from samples and PLA2-HRP conjugate compete for the anti-PLA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLA2 from the sample, fewer sites are left to bind PLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLA2 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001011614.1
ncbi gb acc num :
NM_001011614.1
ncbi mol weight :
19,058 Da