ELISA/assay

Guinea Pig Apolipoprotein D ELISA Kit

MBS720712

48-Strip-Wells

470 USD

ELISA Kit

Guinea Pig Apolipoprotein D ELISA Kit

Apolipoprotein D

apolipoprotein D; Apolipoprotein D; apolipoprotein D; apo-D; apolipoprotein D

APOD

APOD; APOD; Apo-D; ApoD

APOD_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Neurobiology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This APOD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig APOD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY APOD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APOD antibody and an APOD-HRP conjugate. The assay sample and buffer are incubated together with APOD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOD concentration since APOD from samples and APOD-HRP conjugate compete for the anti-APOD antibody binding site. Since the number of sites is limited, as more sites are occupied by APOD from the sample, fewer sites are left to bind APOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOD concentration in each sample is interpolated from this standard curve.

4502163

NP_001638.1

NM_001647.3

P05090

21,276 Da

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]

Function: APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Subunit structure: Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2. Ref.9. Subcellular location: Secreted. Tissue specificity: Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Post-translational modification: N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor). Miscellaneous: APOD is primarily localized in HDL (60-65%), with most of the remainder in VHDL and only trace amounts in VLDL and LDL. Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

48-Strip-Wells

470 USD

96-Strip-Wells

675