catalog number :
MBS720705
products type :
ELISA Kit
products full name :
Rabbit Calcitonin Gene Related Peptide ELISA Kit
products short name :
Calcitonin Gene Related Peptide
other names :
calcitonin gene-related peptide isoform 3 preproprotein; Calcitonin gene-related peptide; calcitonin; calcitonin gene-related peptide; calcitonin-related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; calcitonin
products gene name :
CGRP
other gene names :
CALCA; CALCA; CALC; CGRP; CALC; CGRP
uniprot entry name :
CALCA_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of CGRP. No significant cross-reactivity or interference between CGRP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CGRP and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This CGRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CGRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the assay: CGRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CGRP antibody and an CGRP-HRP conjugate. The assay sample and buffer are incubated together with CGRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CGRP concentration since CGRP from samples and CGRP-HRP conjugate compete for the anti-CGRP antibody binding site. Since the number of sites is limited, as more sites are occupied by CGRP from the sample, fewer sites are left to bind CGRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CGRP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001258895.1
ncbi gb acc num :
NM_001271966.1
ncbi mol weight :
15,308 Da