ELISA/assay

Mouse Activating Transcription Factor 6 ELISA Kit

MBS720701

48-Strip-Wells

470 USD

ELISA Kit

Mouse Activating Transcription Factor 6 ELISA Kit

Activating Transcription Factor 6

ATF6; Cyclic AMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; activating transcription factor 6; Activating transcription factor 6 alpha; ATF6-alpha

ATF6

ATF6; ATF6; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha

ATF6A_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ATF-6. No significant cross-reactivity or interference between ATF-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF-6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ATF-6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ATF-6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: ATF-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF-6 antibody and an ATF-6-HRP conjugate. The assay sample and buffer are incubated together with ATF-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF-6 concentration since ATF-6 from samples and ATF-6-HRP conjugate compete for the anti-ATF-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF-6 from the sample, fewer sites are left to bind ATF-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF-6 concentration in each sample is interpolated from this standard curve.

2245630

AAB64434.1

74,585 Da

ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Metabolism Of Proteins Pathway (106230); PERK Regulates Gene Expression Pathway (105907); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5 -CCAC[GA]-3 half of the ER stress response element (ERSE) (5 -CCAAT-N(9)-CCAC[GA]-3 ) and of ERSE II (5 -ATTGG-N-CCACG-3 ). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily. Protein type: Membrane protein, integral; Transcription factor. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus. Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; protein folding; transcription, DNA-dependent; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction. Disease: Achromatopsia 7

48-Strip-Wells

470 USD

96-Strip-Wells

675