ELISA/assay

Mouse Ferritin light chain (FTL) ELISA Kit

MBS7206974

48-Strip-Wells

470 USD

ELISA Kit

Mouse Ferritin light chain (FTL) ELISA Kit

Ferritin light chain (FTL)

ferritin light chain; Ferritin light chain; ferritin light chain; ferritin L-chain; ferritin L subunit; ferritin light polypeptide-like 3; ferritin, light polypeptide

FTL

FTL; FTL; LFTD; NBIA3; Ferritin L subunit

FRIL_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FTL. No significant cross-reactivity or interference between FTL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FTL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This FTL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FTL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: FTL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FTL antibody and an FTL-HRP conjugate. The assay sample and buffer are incubated together with FTL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTL concentration since FTL from samples and FTL-HRP conjugate compete for the anti-FTL antibody binding site. Since the number of sites is limited, as more sites are occupied by FTL from the sample, fewer sites are left to bind FTL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTL concentration in each sample is interpolated from this standard curve.

20149498

NP_000137.2

NM_000146.3

20,020 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Integrated Pancreatic Cancer Pathway (711360); Iron Uptake And Transport Pathway (187191); Membrane Trafficking Pathway (106160); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Scavenging By Class A Receptors Pathway (833814); Transmembrane Transport Of Small Molecules Pathway (106572)

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

FTL: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS). It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3); also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. Belongs to the ferritin family. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: ferritin complex; membrane; cytosol. Molecular Function: identical protein binding; protein binding; ferric iron binding; iron ion binding. Biological Process: receptor-mediated endocytosis; iron ion homeostasis; cellular iron ion homeostasis; post-Golgi vesicle-mediated transport; iron ion transport; transmembrane transport. Disease: Hyperferritinemia With Or Without Cataract; L-ferritin Deficiency; Neurodegeneration With Brain Iron Accumulation 3

48-Strip-Wells

470 USD

96-Strip-Wells

675